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Round Robin test on bio-imaging transfer standard for 3D optical profilers

Optical Metrology
R&D Engineering Manager, Ph.D. in Optical Engineering, MSc in Photonics at Sensofar Metrology | Other articles

Carlos has collaborated since 2010 on the development of confocal, interferometry and focus variation technologies at Sensofar, where he holds the R&D Engineering Manager position since 2018. His interests are Optomechanical Systems Design and Image Processing.
Its consolidated research work in optical engineering confer the Sensofar R&D group an outstanding position to always stay up-to-date in terms of innovation and the highest technological level.

Round Robin test on bio-imaging transfer standard for 3D optical profilers, full article
A. Nolvi,1,2 T. Viitala,1 A. García Pérez,1 N. Sandler,3 E. Hæggström,1 C. Bermudez,4 R. Artigas,4 I. Kassamakov1
1Univ. of Helsinki (Finland)
2Åbo Akademi (Finland)
3Åbo Akademi Univ. (Finland)
4Sensofar Tech SL, (Spain)

Proceedings Volume 10110, Photonic Instrumentation Engineering IV; 101100M (2017)
Event: SPIE OPTO, 2017, San Francisco, California, United States

Abstract

A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test on bio-imaging, aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S neox type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average.

All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.

surface texture and coating thickness